Journal: JACC: Basic to Translational Science

Article Title: Ubiquitin Specific Protease 9X Regulates the Activation of ARK5 and Promotes Progression of Fibrotic Remodeling

doi: 10.1016/j.jacbts.2025.02.014

Figure Lengend Snippet: ARK5 Facilitates TGF-β1–Induced Myofibroblast Transformation via Activating RhoA/MRTF-A Pathway (A) Fibroblasts were infected with USP9X siRNA, then subjected to TGF-β1 stimulation. Total RNA samples were collected and sequenced. The most significantly altered KEGG pathways were displayed. (B) RhoA activity was assessed using western blot analysis after transfecting CFs with ARK5 siRNA or siRNA-control. (C) CFs were infected with siRNA-control, siRNA-USP9X, Ad-GFP, or Ad-ARK5, and then tested for RhoA activity. (D) MRTF-A protein levels in the nuclear and cytosolic fractions were detected by western blot analysis. (E) Immunofluorescence staining was performed to detect the intensity of MRTF-A. Scale bar: 50 μm. (F) CFs were infected with siRNA-control, siRNA-USP9X, Ad-GFP, or Ad-ARK5, and then tested for MRTF-A protein levels. (G) Representative immunofluorescence staining of MRTF-A for each group. Scale bar: 50 μm. n = 3-5, Values represent the mean ± SEM. One-way analysis of variance followed by Tukey’s post hoc test was used for statistical analysis. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. Abbreviations as in , , and .

Article Snippet: Antibodies against USP9X (55054-1-AP), α-SMA (14395-1-AP), mechanosensitive transcription factor myocardin-related transcription factor-A (MRTF-A) (21166-1-AP), Ubiquitin (10201-2-AP), glyceraldehyde 3-phosphate dehydrogenase (60004-1-Ig), PCNA (10205-2-AP), and RhoA (10749-1-AP) were purchased from Proteintech.

Techniques: Transformation Assay, Infection, Activity Assay, Western Blot, Control, Immunofluorescence, Staining

Journal: Pharmaceutics

Article Title: A Nanoliposome Platform Co-Delivery of Hydroxypinacolone Retinoate and Carnosine for Enhanced Epidermal/Dermal Delivery and Multi-Functional Anti-Aging Efficacy

doi: 10.3390/pharmaceutics18040454

Figure Lengend Snippet: In vitro skin permeability of Free-HC and HC-NLPs. ( A ) Cumulative skin permeation and ( B ) retention of CA over 24 h. ( C ) Cumulative skin retention of HPR. && p < 0.01 vs. Free-HC. Mean ± SD, n = 3. ( D ) Fluorescence microscopy images showing the penetration of RhoB (red) in porcine skin over 4 h. Scale bar: 200 μm.

Article Snippet: 4′,6-Diamidino-2-phenylindole (DAPI) and Ras Homolog Family Member B (RhoB) were purchased from MedChemExpress (MCE, Monmouth Junction, NJ, USA).

Techniques: In Vitro, Permeability, Fluorescence, Microscopy

Journal: Pharmaceutics

Article Title: A Nanoliposome Platform Co-Delivery of Hydroxypinacolone Retinoate and Carnosine for Enhanced Epidermal/Dermal Delivery and Multi-Functional Anti-Aging Efficacy

doi: 10.3390/pharmaceutics18040454

Figure Lengend Snippet: Cellular uptake of Free-RhoB and RhoB-NLPs in HDF cells. CLSM images showing intracellular RhoB fluorescence after 2 h and 4 h incubation. Scale bar: 50 μm.

Article Snippet: 4′,6-Diamidino-2-phenylindole (DAPI) and Ras Homolog Family Member B (RhoB) were purchased from MedChemExpress (MCE, Monmouth Junction, NJ, USA).

Techniques: Fluorescence, Incubation